Slanetz & Bartley Agar, 90mm Plate

Description

9098 – Slanetz & Bartley Agar, 90mm Plate is a ready-to-use prepared plate supplied by SGL for detection and enumeration of intestinal enterococci in water and environmental samples. It is suitable for use in water testing; environmental testing and food/beverage laboratories. This product is relevant to microbiology workflows described in ISO 7899-2 intestinal enterococci in water and ISO 11133 Microbiology of food, animal feed and water.

Slanetz & Bartley is a medium used for the enumeration of enterococci in water and other liquids. The medium was originally designed for membrane filtration but has proved useful as a direct plate medium.

Enterococci reduce tetrazolium chloride to formazan which produces dark red/maroon appearance of the colonies, though not all species reduce TTC so paler colonies should be considered. Gram negative bacteria are inhibited by sodium azide.

Testing methods may vary depending on the sector using the medium.

1. Water Testing uses the membrane filtration technique. The sample is filtered through the membrane then placed on the surface of the agar, The plate is then incubated for 4hours at 35-37°C to recover stressed organisms then for a further 44-48hours at 44-45°C.
2. Food Laboratories uses the direct plate method. The sample is homogenised in physiological saline then spread onto the plate. The plate is then incubated for 48hours at 35-37°C

Confirmation may be necessary by aesculin hydrolysis using Kanamycin Aesculin Azide Agar (KAAA).

Tryptose provides nitrogen, minerals and amino acids where yeast extract provides vitamins. Glucose is the carbon source and dipotassium phosphate buffers the medium.

SKU: 9098