Selenite Cystine Broth, Universal

Description

9035 – Selenite Cystine Broth, Syrup is a ready-to-use bottled medium supplied by SGL for routine microbiological testing. It is suitable for use in food and beverage and clinical laboratories. Manufactured and quality controlled to SGL internal specifications.

Laboratories use Selenite Cystine Broth as a selective, enrichment liquid medium to isolate and recover Salmonella species from environmental, foodstuffs (including animal feed) and clinical (faecal) samples. It is used especially for the detection of S. typhi and S. paratyphi.

The medium relies on the ability of Salmonella to develop in the presence of selenite which inhibits most other bacteria.

Enzymatic digest of casein and enzymatic digest of animal tissue provide nitrogenous substances and amino acids. Sodium phosphate buffers the medium to stabilise pH and reduce selenite toxicity. Lactose provides an energy source, whilst helping to maintain optimal pH conditions. Selenite reducing bacteria produce alkali which increases pH, whereas acid produced from lactose fermentation lowers pH. This helps the maintenance of a neutral, or slightly decreased pH.

Sodium selenite inhibits Gram positive bacteria and suppresses most Gram negative enterics other than Salmonella.  L-cystine is considered to enhance the recovery of Salmonella by the reduction of toxicity.

The medium is light and temperature sensitive, and if overheated during production may form a brick red precipitate.

SGL manufactures the product in compliance with ISO 9001 (reference FM37824). A UKAS-accredited laboratory operating to ISO/IEC 17025 (accreditation number 4356) tests the product in accordance with ISO 11133. At the customer’s request, the laboratory can also perform additional specification testing.

SKU: 9035