Legionella GVPC Medium (ISO), 90mm Plate

Glycine Vancomycin Polymyxin Cycloheximide (GVPC) agar is a medium recommended by ISO 11731:2017 for the isolation of Legionella and estimation of their numbers in water samples. The medium is identical to Buffered Charcoal Yeast Extract (BCYE) agar except for the addition of the four selective compounds.

Yeast extract provides sources of nitrogen, carbon and vitamins. Activated charcoal neutralises toxic metabolic by-products and ACES and potassium hydroxide buffer the media to the optimum pH. L-Cysteine and Iron (III) pyrophosphate are essential growth factors and α-Ketoglutarate stimulates growth. Glycine and the three antibiotics provide effective selectivity against non-target organisms and agar is the gelling agent. This medium conforms to the formulation and performance requirements of ISO 11731:2017.

Specifications

Container 90mm Plate
Pack Size 10
Shelf Life 26 weeks
Storage Temp 2-8oC
Appearance Black, opaque gel
Recommended Incubation 37oC
pH 6.9 +/-0.2
Fill Volume 20ml

Quality Control

Organisms Ref. No Result
Legionella pneumophila NCTC 12821 >70% recovery compared to control. White colonies
Legionella pneumophila NCTC 11192 >70% recovery compared to control. White colonies
Legionella pneumophila Wild strain >50% recovery compared to control. White colonies
Enterococcus faecalis ATCC 19433 NCTC 775 Total inhibition
Escherichia coli ATCC 8739 NCIB 8545 <10% growth compared with control plate
Staphylococcus aureus ATCC 6538 NCTC 10788 <10% growth compared with control plate
Pseudomonas aeruginosa ATCC 27853 NCTC 12934 Total or partial inhibition
Legionella anisa ATCC 35292 >70% recovery compared to control. White colonies

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Legionella GVPC Medium (Glycine Vancomycin Polymyxin Cycloheximide) is a medium recommended by ISO 11731:2017 for the isolation of Legionella and estimation of their numbers in water samples. The medium is identical to Buffered Charcoal Yeast Extract (BCYE) agar except for the addition of the four selective compounds.

Yeast extract provides sources of nitrogen, carbon and vitamins. Activated charcoal neutralises toxic metabolic by-products and ACES and potassium hydroxide buffer the media to the optimum pH. L-Cysteine and Iron (III) pyrophosphate are essential growth factors and α-Ketoglutarate stimulates growth. Glycine and the three antibiotics provide effective selectivity against non-target organisms and agar is the gelling agent. This Legionella GVPC Medium conforms to the formulation and performance requirements of ISO 11731:2017.

Shelf Life 26 weeks from manufacture