Specifications
Container | 90mm Plate |
Pack Size | 10 |
Shelf Life | 26 weeks |
Storage Temp | 2-8oC |
Appearance | Black, opaque gel |
Recommended Incubation | 37oC |
pH | 6.9 +/-0.2 |
Fill Volume | 20ml |
Legionella GVPC Selective Agar – Glycine Vancomycin Polymyxin Cycloheximide (GVPC) agar is a medium recommended by ISO 11731:2017 for the isolation of Legionella and estimation of their numbers in water samples. The medium is identical to Buffered Charcoal Yeast Extract (BCYE) agar except for the addition of the four selective compounds.
Yeast extract provides sources of nitrogen, carbon and vitamins. Activated charcoal neutralises toxic metabolic by-products and ACES and potassium hydroxide buffer the media to the optimum pH. L-Cysteine and Iron (III) pyrophosphate are essential growth factors and α-Ketoglutarate stimulates growth. Glycine and the three antibiotics provide effective selectivity against non-target organisms and agar is the gelling agent. This medium conforms to the formulation and performance requirements of ISO 11731:2017.
Legionella GVPC agar plates are available in packs of 10 with a shelf life of 26 weeks. Legionella selective agar is applicable to the water & environmental monitoring sectors.
Container | 90mm Plate |
Pack Size | 10 |
Shelf Life | 26 weeks |
Storage Temp | 2-8oC |
Appearance | Black, opaque gel |
Recommended Incubation | 37oC |
pH | 6.9 +/-0.2 |
Fill Volume | 20ml |
Organisms | Ref. No | Result |
---|---|---|
Legionella pneumophila | NCTC 12821 | >70% recovery compared to control. White colonies |
Legionella pneumophila | NCTC 11192 | >70% recovery compared to control. White colonies |
Legionella pneumophila | Wild strain | >50% recovery compared to control. White colonies |
Enterococcus faecalis | ATCC 19433 NCTC 775 | Total inhibition |
Escherichia coli | ATCC 8739 NCIB 8545 | <10% growth compared with control plate |
Staphylococcus aureus | ATCC 6538 NCTC 10788 | <10% growth compared with control plate |
Pseudomonas aeruginosa | ATCC 27853 NCTC 12934 | Total or partial inhibition |
Legionella anisa | ATCC 35292 | >70% recovery compared to control. White colonies |
8068
SKU: 8068