Specifications
| Appearance | Pale Orange Gel |
| Container | 90mm Plate |
| Fill Volume | 18ml |
| Pack Size | 10 |
| Box/Case Size | 10x10 (100 Plates) |
| Shelf Life | 12 Weeks |
| Storage Temp | 2-8°C |
Burkholderia Cepacia Selective Agar (BCSA), USP
Burkholderia Cepacia Selective Agar USP, a selective medium for the isolation of Burkholderia cepacia with the formulation specified in USP <60> Microbiological Examination of Non-sterile Products – Tests for Burkholderia cepacia Complex.
Description
7731 – Burkholderia Cepacia Selective Agar (BCSA), USP is a ready-to-use prepared plate supplied by SGL for routine microbiological testing. It is suitable for use in pharmaceutical microbiology; environmental monitoring; and clinical testing. Manufactured and quality controlled to SGL internal specifications.
Burkholderia Cepacia Selective Agar (BCSA) USP is a differential and selective medium for the isolation of Burkholderia cepacia with the formulation specified in USP <60> Microbiological Examination of Non-sterile Products.
Burkholderia cepacia is a Gram positive, aerobic bacteria capable of surviving for extended periods in hostile environments due to its innate resistance to many antibiotics and antiseptics. This is due to the organisms ability to produce biofilms. It is recognised as an important pathogen affecting patients with cystic fibrosis. Isolation and identification of the organism is important to expedite correct treatment, verify carrier status and maintain infection control.
Yeast extract and peptone provide nutritional components, sodium chloride gives the medium osmotic balance. Agar is the solidifying agent giving a stable, solid surface for the organism to grow. Antimicrobics must be added (for example; crystal violet, gentamycin, polymixin B and vancomycin) to inhibit micro-organisms other than Burkholderia cepacia complex (BCC) found in respiratory secretions.
Acid production from carbohydrate oxidation produce a yellow zone in the medium. This is a typical positive reaction to BCC, whilst peptone utilisation produces a pink zone. Colonies that absorb the crystal violet dye will appear purple to purple-grey. Growth may require 48-72 hours aerobically at 30-35°C.
SGL manufactures and quality-controls the product to its internal specifications and in compliance with ISO 9001 (reference FM37824). A UKAS-accredited laboratory operating to ISO/IEC 17025 (accreditation number 4356) tests the product. At the customer’s request, the laboratory can also perform additional specification testing.
Pre-Poured Burkholderia Cepacia Selective Agar (BCSA)
SKU: 7731
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