Mannitol Selenite Broth, Universal

Selenite Mannitol Broth is an alternative to Selenite F Broth for the selective enrichment of Salmonellae spp from Clinical, Food and Environmental specimens. Based on Buffered Mannitol Broth, it is made selective by the addition of Sodium Biselenite. The fermentation of Mannitol by Salmonellae is said to correct the alkaline pH swing which can occur during incubation. Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media.

Specifications

Container Universal
Available fill size(s) (ml) 10
Neck width (mm) 28
Caps PP, Perf, Metal
Shelf life 7 months
Storage Temp 2-8oC
Appearance clear, colourless to slightly yellow or pink
Recommended Incubation 37oC
pH 7.1 +/-0.2

Quality Control

Organisms Ref. No Result
Salmonella typhimurium ATCC 14028 Recovery of Salmonella from mixed cultures containing a predominance of coliforms. Semi-confluent or confluent growth overnight from an inoculum of <100cfu
Escherichia coli ATCC 8739 NCIB 8545 Poor/no growth (<10% recovery)
Klebsiella aerogenes NCTC 9528 Poor/no growth (<10% recovery)

Supporting Documents

MSDS Download
Technical data sheet Download

Description

0594

SKU: 0594